Biotechnological Aspects of Production, Characterization and Application of Keratinase

Authors

  • Sadia Azaz  Institute of Industrial Biotechnology (IIB) Govt. College University Lahore, Pakistan
  • Sikander Ali  Institute of Industrial Biotechnology (IIB) Govt. College University Lahore, Pakistan
  • Afaq Ahmed  Institute of Industrial Biotechnology (IIB) Govt. College University Lahore, Pakistan
  • Maryam Javid  Institute of Industrial Biotechnology (IIB) Govt. College University Lahore, Pakistan

Keywords:

Keratinophilic fungi, Streptomyces, actinomyces, serine protease, ubiquitin proteasome pathway, bio-hydrogen.

Abstract

Keratin is insoluble biopolymer and mainly present in tissues of birds, amphibians, reptiles and mammals. Many industries use keratin as raw material and accumulation of keratin causes many environmental problems. To reduce keratin accumulation there should be a need to develop an easy and cheap process for environmental remediation of keratin. Microbial keratinase, a proteolytic enzyme, is used to reduce pollution and is mainly obtained from Keratinophilic fungi, bacteria and actinomyces and Streptomyces. Keratin is degraded in prokaryotes by degrading serine protease and in eukaryotes by ubiquitin (Ub)-proteasome pathway (UPP). Keratinase used to degrade feathers of poultry and converted into high energy input feather meal, in leather industry for management of wastes which mainly causes the water pollution, by producing bio hydrogen from solid wastes. In this review it is investigated that microbial keratinase is less expensive than conventional production of keratinase and have many advantages to reduce pollution.

References

  1. Anbu P., A. Hilda and S.C.B. Gopinath. 2004. Keratinophilic fungi of poultry farm and feather dumping soil in Tamil Nadu, India.Mycopathologia., 158(3): 303–309.
  2. Balint, B. Z., Bagi, G. Rakhley, K. Perei and K. L. Kovacs. 2005. Utilization of keratin containing bio waste to produce biohydrogen. Appl. Microbiol. Biotechnol., 69 : 404-410.
  3. Benemann, J.1996. Hydrogen biotechnology: progress and prospects. Nat. Biotechnol., 14:1101-1103.
  4. Bockle, B., B. Galunski and R. Muller. 1995. Characterization of a keratinolytic serine protease from Streptomyces pactum DSM40530. Appl. Environ. Microbiol., 61:3705-3710.
  5. Bodde, S.G., M.A. Meyer and J. McKittrick. 2011. Correlation of the mechanical and structural properties of cortical rachis keratin of rectrices of the Toco Toucan (Ramphastos toco). J. Mech. Behav. Biomed. Mater., 4:723–732.
  6. Brandelli, A. D., J. Daroit and A. Riffel. 2010. Biochemical features of microbial keratinases and their production and applications. Appl. Microbiol. Biotechnol., 85:1735.
  7. Brutt, E. H. and J. M.Ichida. 1999. Bacteria useful for degrading keratin. US patent 621467.
  8. Cai, C., G.B.G. Lou and X.D. Zheng. 2008. Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis. J. Zhejiang University Sci. B., 9(1): 60–67.
  9. Cammack, R., M.Frey, and R. Robson. 2001. Hydrogen as a fuel: learning from nature. Taylor and Francis, London.,
  10. Cherry, J. P., C. T. Young and A. L Shewfelt.1975. Characterization of protein isolates from keratinous material of poultry feathers. J. Food. Sci., 40: 331-335.
  11. Ciechanover, A. and A. L. Schwart.1998. The ubiquitin proteasome pathway: the complexity and myriad functions of protein death. Proc. Natl. Acad. Sci. USA., 95: 2727-2730.
  12. Cortezi, M.J., C.J.B. Contiero, de Lima, R. B. Lovaglio, and R. Monti. 2008. Characterization of a feather degrading by Bacillus amyloliquefaciens protease: a new strain, World. J. Agri. Sci.,4(5):648–656.
  13. Elmayergi, H. H. and R. E.Smith. 1971. Influence of growth of Streptomyces fradie on pepsin HCl digestibility and methionine content of feather meal. Can. J. Microbiol., 17: 1067-1072.
  14. Fortierand, A.M. and M. Cadrin. 2012. Simple epithelial keratins k8 and k18: from structur altoregulatory protein in Keratin Structure, Properties and Applications. R.Dullaart and J.Mousqu`es., 1–35.
  15. Ganaie, M.A., S. Sood, G. Rizvi, and T.A. Khan. 2010. Isolation and identification of keratinophilic fungi from different soil samples in Jhansi City (India). Plant Pathol. J., 9(4): 194–197.
  16. Gehring, A. G. 2002. Unhairing with proteolytic enzymes derived from Streptomyces griseus. J. Am. Leather. Chem. Assoc., 97: 406-411.
  17. Glickman, M. H., D. M. Rubin, O. Coux, I. Wefes, G. Pfeifer, Z .Cjeka, W. Baumeister, V. A. Fried and D. A Finely.1998. A subcomplex of proteasome regulatory particle required for ubiquitin conjugate degradation and related to the cop 9 signalosome and eif3. Cell., 94: 615-23.
  18. Goldberg, A. L. 2003. Protein degradation and protection against misfolded or damaged proteins. Nature, 426: 895-99.
  19. Grazziotin, A. F., A. Pimentel, E. V. De-Jong, and A. Brandelli. 2006. Nutritional improvement of feather protein by treatment with microbial keratinase. Anim. Feed. Sci. Technol., 126: 135-44.
  20. Groll, M., L. Ditzel, J. Lowe, D. Stock, M. Bochtler, H. D. Bartunik, and R. Huber.1997. Structure of 20S proteasome from yeast at 2.4°A resolution. Nature, 386: 463-471.
  21. Gugnani, H. C., S. Sharma, B. Gupta, and S. Gaddam. 2012. Prevalence of keratinophilic fungi in soils of St. Kitts and Nevis. Journal of Infection in Developing Countries., 6(4): 347–351.
  22. Gupta, R. and P. Ramnani. 2006. Microbial keratinases and their prospective applications: an overview. Appl. Microbiol. Biotechnol., 70(1): 21–33.
  23. Korkmaz., H. Hur and S. Dincer. 2004. Dincer,Characterization of alkaline keratinase of Bacillus licheniformis strainHK-1from poultrywaste. Annals of Microbiology., 54(2) 201–211.
  24. C. Gugnani. 2000. Non-dermatophytic filamentous keratinophilic fungi and their role in human infections in Biology of Dermato- phytes and Other Keratinophilic Fungi. J. Guarro., 109–114.
  25. Harrap, B. S. and E. F Woods.1964. Soluble derivatives of feather keratin, isolation, fractionation and amino acid composition. Biochem. J., 192: 8-18.
  26. Hoq, M. M., K. A. Z. Siddiquee, H. Kawasaki and T. Seki. 2005. Keratinolytic activity of some newly isolated Bacillus species. J. Biol. Sci., 5 (2): 193–200.
  27. Jeevana, P., C.M. Kumari and V.V. Lakshmi. 2013. Efficient degradation of feather by keratinase producing Bacillus sp. Intern. J. Microbiol.,1-7.
  28. Joshi, S. G., M. M. Tejashwini, N. Revati, R. Sridevi, and D. Roma. 2007. Isolation, identification and characterization of a feather degrading bacterium. Intern. J. Poultry Sci., 6(9): 689–693.
  29. Kachuei, R. M., B. Emami, Naeimi, and K. Diba. 2012. Isolation of keratinophilic fungi from soil in Isfahan province,Iran. J. deMycologie Medicale., 22(1): 8–13.
  30. Kanchana, R. 2012. Farm waste recycling through microbial kerati- nases. J. Appl. Sci. Environment. Sanitation., 7(2): 103–108.
  31. Kisselev, A. F., T. N.Akopian, K. M. Woo, and A. L Goldberg. 1999. Implications for understanding the degradative mechanism and antigen presentation. J. Biol. Chem., 274: 3363-3371.
  32. Korni??owicz, T. and J. Bohacz. 2011. Biodegradation of keratin waste: theory and practical aspects. Waste Manag., 31(8): 1689–1701.
  33. Kumar, R., S. Mishra, Maurya and H.B.Sahu. 2013. Isolation and identification of keratinophilic fungi from garbage waste soils of Jharkh and region of India. Europ. J. Expl. Biol., 3(3): 600–604.
  34. Lee, G. G., P. R. Ferket and J. C. Shih. 1991. Improvement of feather digestibility by bacterial keratinase as a feed additive. FASEB. J., 59: 1312-15.
  35. Lin, X. C., Lee, E.S. Casale and J.C. Shih, 1992. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environ. Microbiol., 58:3271–3275.
  36. Lin, X. D., W. Kelemen, E. S. Miller and J. C. H Shih.1995. Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. Appl. Environ. Microbiol., 61: 1469-74.
  37. Lowe, J. D., B. Stock, P. Jap, Zwickl , W. Baumeister and R. Huber. 1995. Crystal structures of the 20S proteasome from the archeon T acidophilum at 3.4°A resolution. Science, 268: 533-39.
  38. Marsal, A.1999. Oxidizing unhairing process with hair recovery. Part I. Experiments on the prior hair immunization. J. Soc. Leather. Technol. Chem., 83: 310-315.
  39. Mart?nez, A.L. and C. Velasco. 2012. Keratin fibers from chicken feathers: structure and advances in polymer composites in Keratin: Structure, Properties and Applications. R. Dullaart and J. Mousqu`es., 149–211.
  40. Matikeviciene, V., D. Masiliuniene and S. Grigiskis. 2009. Degradation of keratin containing wastes by bacteria with keratinolytic activity in Proceedings of the 7th International Scientific and Practical Conference. 1:284-289.
  41. McKittrick, J., P.Y. Chen, S.G. Bodde, W. Yang, E.E. Novitskaya, and M.A. Meyer’s. 2012. The structure, functions, and mechanical properties of keratin. JOM., 64: 449–468.
  42. Meyers, M.A., P. Chen, A.Y. Lin, Y .Seki. 2008. Biological materials: structure and mechanical properties. Prog. Mater. Sci., 5(31): 206.
  43. Molyneux, G. 1959. The digestion of wool by a keratinolytic Bacillus. Aust. J. Biol. Sci., 12: 274–281.
  44. Onifade, A. A., N. A.Al-Sane, A. A. Al- Musallam, and S .Al-Zarban. 1998. Potentials for biotechnological applications of keratin degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol., 66: 1- 11.
  45. Pakshir, K., M.R. Ghiasi, K. Zomorodian and A.R. Gharavi. 2013. Isolation and molecular identification of keratinophilic fungi from public parks soil in Shiraz, Iran. BioMed Research International., 6: 1-5.
  46. Papadopoulos, M. C., A. R. El-Boushy, A. E. Roodbeen and E. H. Ketelaars.1986. Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal. Anim. Feed. Sci. Technol., 14: 279-290.
  47. Pickart, C. M. 2001. Mechanisms underlying ubiquitination. Annu. Rev. Biochem., 70: 503-533.
  48. Ramasami, T., B. U. Nair, P. Thanikaivelan and J. R Rao.1999. Beam house and tanning operations: process chemistry revisited. J. Soc. Leather. Technol. Chem., 83: 39-45.
  49. Ramasami, T., P. Thanikaivelan, B. U. Nair and J. R. Rao. 1998. Emerging leather processing strategies for waste minimization. Buljan. J., 97-183.
  50. Ramesh, V.M. and A. Hilda.1998. Incidence of keratinophilic fungi in the soil of primary schools and public parks of Madrascity, India. Mycopathologia, 143(3): 139–145.
  51. Rao, J. R., B. U. Nair and P. Thanikaivelan.1997. Isolation and characterization of low affinity chromium (III) complex in chrome tanning solutions. J. Soc. Leather. Technol. Chem., 81: 234-38.
  52. Riffel, A. and A. Brandelli. 2006. Keratinolytic bacteria isolated from feather waste. Braz. J. Microbiol., 37: 395-99.
  53. Rogel, M. R., A. Jaitovich and K. M. Ridge. 2010. The role of the ubiquitin proteasome pathway in keratin intermediate protein filament protein degradation. Proc. Am. Thoracic. Soc., 7: 71-76.
  54. Shadzi, S. M., Chadeganipour and M. Alimoradi. 2002. Isolation of keratinophilic fungi from elementary schools and public parks in Isfahan, Iran. Mycoses., 45(11-12): 496–499.
  55. Shih, J. C. H. 1993. Recent development in poultry waste digestion and feather utilization-A review. Poul. Sci., 72: 1617-1620.
  56. Shih, J. C. H., and C. M Williams.1990. Feather lysate, a hydrolysed feather feed ingredient and animal feeds containing the same. Poul. Sci.,1.
  57. Tamura, N., F. Lottspeich, W. Baumeister and T Tamura.1998. The role of tricorn protease and its amino peptidase interacting factors in cellular protein degradation. Cell., 95: 637-48.
  58. Thanikaivelan, P. 2002. Zero discharge tanning: a shift from chemical to biocatalytic leather processing. Environ. Sci. Technol., 36: 4187-4194.
  59. Thanikaivelan, P., J. R. Rao, B. U. Nair and T. Ramasami. 2004. Progress and recent trends in biotechnological methods for leather processing. Trends Biotechnol., 22: 181-188.
  60. Wang, X. and C. M Parsons. 1997. Effect of processing systems on protein quality of leather meal and hog hair meals. Poul. Sci., 76: 491-496.

International Journal of Scientific Research in Science and Technology

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2017-06-30

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Volume 3, Issue 4, May-June-2017

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[1]
Sadia Azaz, Sikander Ali, Afaq Ahmed, Maryam Javid, " Biotechnological Aspects of Production, Characterization and Application of Keratinase, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 3, Issue 4, pp.327-336, May-June-2017.